雏鸭感染鹅源H5N1亚型禽流感病毒后免疫器官T细胞免疫功能及IL-2 mRNA转录变化

doi:10.11843/j.issn.0366-6964.2014.09.016

Abstract

Abstract:

Technology of cell culture，cyto-histochemistry staining and real-time fluorescence quantitative PCR （SYBR GreenⅠ） were used to research dynamic changes of the proliferation responses of T-lymphocytes to ConA，the quantity of T_ANAE⁺ cells and the transcription of IL-2 mRNA in immune organs of ducks infected with AIV at 21 days old，which were useful to reveal the mechanism and regulation of immunity.The experiment provided scientific evidence for the mechanism of morbidity and useful effective prevention.The results were as follows：the transcription of IL-2 mRNA，the proliferation responses of T-lymphocytes to ConA and the quantity of T_ANAE⁺ cells in immune organs were all decreased remarkably in initial period of AIV infection.And it indicated that molecular immune function of cytokines was inhibited，and the activation and proliferation of the immunocompetent cells，especially T-lymphocyte，were inhibited as well.The cellular immunity functions were much lower than that of controls，which could be the main reasons of the ducks death in initial period of AIV infection.

CLC Number:

S852.659.5

YU Xue-wu，LYU Xiao-ping，LIU Chao-nan，GAO Xue-li，SHI Lin，LIU Ming，ZHENG Shi-min. Changes of T Lymphocytes Proliferation Function，Quantity of T_ANAE⁺ Cells and Transcription of IL-2 mRNA in Immune Organs of Ducks Infected with H5N1 Subtype Avian Influenza Virus Isolated from Geese[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(9): 1497-1504.

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Changes of T Lymphocytes Proliferation Function，Quantity of T_ANAE⁺ Cells and Transcription of IL-2 mRNA in Immune Organs of Ducks Infected with H5N1 Subtype Avian Influenza Virus Isolated from Geese

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Changes of T Lymphocytes Proliferation Function，Quantity of TANAE+ Cells and Transcription of IL-2 mRNA in Immune Organs of Ducks Infected with H5N1 Subtype Avian Influenza Virus Isolated from Geese

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Changes of T Lymphocytes Proliferation Function，Quantity of T_ANAE⁺ Cells and Transcription of IL-2 mRNA in Immune Organs of Ducks Infected with H5N1 Subtype Avian Influenza Virus Isolated from Geese